LSI Tissue Processing Facility

Before booking, please take the time to read over the following Tissue Processing Facility policies:

Olympus BX61 Motorized Fluorescence Microscope

The BX61 is a fully motorized research microscope built on the platform of the traditional upright frame. All major microscope functions can be completely automated, including focus, illumination, objective lens selection and filter wheels. A complete software command set allows for full computer control.

Cost: $5 per hour

The Olympus BX61 system offers the following:

Olympus DP72 12.8 MP cooled
Colour CCD camera
Olympus cellSens Dimension imaging
Software, including 5D acquisition and Automated Full Count & Measure modules.
Super Apochromatic objective lenses – 4x, 10x, 20x, 40x, 60x oil, 100x oil Internal motorized Z-drive for automated Z-stacks and
Extended Focus Imaging (EFI)
6 position motorized fluorescence
Turret for automated multichannel fluorescence acquisition
6 position motorized nose-piece
8 position manual condenser
Right hand manual XY stage
Trinocular observation tube
Differential Interference Contrast
(DIC) optics
Exfo x-Cite metal halide fluorescence
Light source with integrated iris
Semrock hard-coated Zero Pixel
Filter cubes – DAPI, FITC, TRITC, Texas Red Imaging computer workstation with high performance wide-screen 24″ LCD

Thermo Scientific Microm HM 525 Cryostat

The LSI Thermo Scientific HM 525 Cryostat is a semi-electronic cryostat with stepper motor advance that provides superior consistency and reliability of sectioning results.

Cost: $5 per hour

Microm HM 525 Cryostat offers the following:

Open top cryostat with stainless steel cooling chamber.
Variable temperature regulation with automatic defrost cycle and integrated fast freezing device down to max -60°C.
Sliding window with heater and fluorescent lamp for cryo-chamber.
Motorized coarse feed in two directions with limit indication and automated switch off at front and rear limits of horizontal travel.
Section counter and indication of sum of section thickness.
Chamber temperature control:+5°C to -35°C
Fast freezing station: max -60°C

Microtome Features:

Section thickness: 1 to 500µm
Fine Section thickness: 1 to 20µm
Trimming thickness: 10 to 500 µm
Specimen retraction during return travel: 40 µm
Vertical specimen stroke: 60 mm
Horizontal specimen movement: 28 mm

Specimen Orientation:

x- and y- axes: universal 8° with zero positioning
z-axis: rotatable 360°

Contact Information:

Instrument Use and Training

Facility room #: 3435
Megan Gilmour
Email: meg.gilmour@gmail.com
Phone: 822-1781